TLC directed isolation and in silico analysis of antimicrobial metabolite from Nigrospora sphaerica inhabiting Croton bonplandianus Baill

N.Chandra Mohana^a, D. Rakshith^a,b, K.P. Ramesha^a, B.R. Nuthan^a, B.P. Harini^c, S. Satish^a

Microbial Drugs Laboratory, Department of Studies in Microbiology, Manasagangotri, University of Mysore, Mysore 570006, Karnataka, India.

Abstract

TLC-bioautography amalgamated with hyphenated spectroscopy aid in precise in situ detection of secondary metabolites with pharmaceutical significance. TLC bioautography offers efficient and economical strategy in identifying compounds of interest from crude extracts. The present investigation has been focused on detection of antimicrobial metabolite from the culture broth of Nigrospora sphaerica inhabiting Croton bonplandianus Baill. The antimicrobial profiling confirmed the bioactive nigrosporalactone to possess broad-spectrum activity against test human pathogens with minimum inhibitory concentration values in the range 6.25 µg to 100 µg. The in silico studies revealed protein targets 1I01 (E. coli beta-ketoacyl reductase), 1IYL (C. albicans N-myristoyl transferase) had the highest binding score of -6.1 Kcal/mol.

Keywords: Mycoendophyte, TLC, bioautography, antimicrobial, Nigrospora sphaerica, Croton bonplandianus Baill.